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Iwoye Dengue Titẹ Apo Idanwo Acid Nucleic (Ọna iwadii fluorescence PCR)
Ọrọ Iṣaaju
Ohun elo yii jẹ ipinnu fun wiwa titẹ agbara ti kokoro dengue nucleic acid ninu omi ara eniyan tabi awọn ayẹwo pilasima.Ohun elo yii da lori ajẹkù kan pato ni gbogbo genome ti iru ọlọjẹ dengue 1 ~ 4 lati ṣe apẹrẹ awọn alakoko kan pato ati awọn iwadii fluorescent TaqMan fun iru kọọkan, ati rii wiwa iyara ati titẹ ti kokoro dengue nipasẹ PCR fluorescent akoko gidi.
Awọn paramita
* Iru apẹẹrẹ: Omi ara tabi Plasma * Awọn ohun elo elo: ABI 7500 Eto PCR-akoko gidi;Bio-rad CFX96;Roche LightCycler480;SLAN PCR System. * Ibi ipamọ -25 ℃ si 8 ℃ ṣiṣi silẹ ati aabo lati ina awọn oṣu 18Awọn eroja 48T/Apo Awọn eroja akọkọ DENV-Iru ifaseyin adalu, lyophilized 2 tubes Awọn alakoko, awọn iwadii, saarin ifasilẹ PCR, dNTPs, Enzyme, ati bẹbẹ lọ. DENV iṣakoso rere, lyophilized 1 tube Plasmids fun tandem dengue kokoro iru 1-4 wiwa awọn ajẹkù ibi-afẹde Iṣakoso odi (omi mimọ) 1.5ml Omi ti a wẹ Itọsọna olumulo 1 ẹyọkan / Iṣẹ ṣiṣe
• Dekun: Akoko imudara PCR kuru ju laarin iru ọja.• Ifamọ giga & pataki: Ṣe agbega ayẹwo ni kutukutu fun itọju kiakia.• Okeerẹ egboogi-kikọlu agbara.Awọn igbesẹ iṣẹ
