2019-nCoV S-RBD Ohun elo Idanwo Antibody Neutralizing (ọna goolu colloidal)
Ọrọ Iṣaaju
Ni lọwọlọwọ, gbogbo awọn ajesara oludije ti 2019-nCoV ni idagbasoke ile-iwosan ni a nṣakoso nipasẹ abẹrẹ inu iṣan.Ajẹsara inu iṣan tabi intradermal le ja si ifakalẹ to lagbara ti omi ara IgG
Diẹ sii ju awọn oludije ajesara 180, ti o da lori ọpọlọpọ awọn iru ẹrọ oriṣiriṣi wa lọwọlọwọ ni idagbasoke lodi si 2019-nCoV.
Awọn ọlọjẹ S jẹ ibi-afẹde pataki ti yomi ara;
Pupọ ninu awọn ọlọjẹ didoju wọnyi fojusi RBD ti amuaradagba S.
Bii o ṣe le ṣe idajọ ṣiṣe ti ajesara 2019-nCoV? --- Apo Idanwo Antibody Neutralizing
Anfani
Ṣaaju idanwo ajesara
Ṣaaju ki o to ajesara, awọn oludije le ṣe awari antibody yomi ti RBD lati pinnu boya ajesara jẹ pataki;
Pupọ awọn oogun ajesara ni aabo
O le ṣe awari awọn ọlọjẹ didoju ti a ṣe nipasẹ ọpọlọpọ awọn ajesara lori ọja;
Sare ati ki o rọrun
Iṣẹ naa rọrun, ko si wiwa ohun elo ti o nilo, awọn abajade le ṣee gba laarin awọn iṣẹju 15.
Iṣẹ idanimọ
O le ṣe iyatọ si apakokoro yomi ti 2019-nCoV ti a ṣe nipasẹ ajesara 2019-nCoV tabi egboogi ti a ṣejade nipasẹ ikolu 2019-nCoV fun iru awọn ajesara kan, gẹgẹ bi ajesara Viral vector (Non-replicating), ajesara ipilẹ RNA ati ajesara subunit Protein ;
Idanwo ẹjẹ gbogbo
Idanwo ẹjẹ gbogbo jẹ ki iṣẹ naa rọrun diẹ sii;
Dopin ti ohun elo
Pre ajesara
Ṣe ipinnu boya wọn ti ni akoran pẹlu coronavirus tuntun ati boya wọn tun nilo lati jẹ ajesara;
Akoko ajesara
Pinnu boya o munadoko titun ipakokoro ipakokoro ti wa ni iṣelọpọ;
Late ipele ti inoculation
Ni ibamu si agbegbe ajakale-arun ti 2019-nCoV, o daba lati rii aye ti 2019-nCoV didoju egboogi nigbagbogbo ni gbogbo oṣu mẹta.
Awọn eroja
| Awọn eroja | Awọn eroja akọkọ | Oye ikojọpọ (Ipato) | ||
| 1 T/Apo | 20 T/Apo | 50 T/Apo | ||
| Kaadi idanwo | Iwọn idanwo ti o ni goolu colloidal ti o ni aami egboogi-eniyan IgG antibody, goolu colloidal ti o jẹ egboogi-adie IgY antibody, 2019-nCoV S-RBD amuaradagba atunko, Adie IgY agboguntaisan | 1 pc | 20 awọn kọnputa | 50 awọn kọnputa |
| Diluent apẹẹrẹ | 0.01M Phosphate saarin ojutu, 0,5% Tween-20 | 0.5ml | 5ml | 10ml |
Iṣẹ ṣiṣe
| Hecin reagent | Isẹgun serum kokoro idanwo neutralization | Lapapọ | |
| Rere | Odi | ||
| Rere | Rere | 84 | 9 |
| Odi | Odi | 8 | 198 |
| Lapapọ | Lapapọ | 92 | 207 |
| Isẹgun ifamọ | Isẹgun ifamọ | 84/92 91.30% (95% CI: 83.58%)～96.17%) | |
| Isẹgun pato | Isẹgun pato | 198/207 95.65% (95% CI: 91.91%)～97.99%) | |
| Yiye | Yiye | 282/299 94.31% (95% CI: 91.05%)～96.65%) | |
Iṣe Hecin reagent lodi si Ọna Comparator lori awọn ayẹwo omi ara/pilasima.
| Hecin reagent | Isẹgun serum kokoro idanwo neutralization | Lapapọ | |
| Rere | Odi | ||
| Rere | 84 | 8 | 92 |
| Odi | 8 | 199 | 207 |
| Lapapọ | 92 | 207 | 299 |
| Isẹgun ifamọ | 84/92 91.30% (95% CI: 83.58%)～96.17%) | ||
| Isẹgun pato | 199/207 96.14% (95% CI: 92.53%)～98.32%) | ||
| Yiye | 283/299 94.65% (95% CI: 91.46%)～96.91%) | ||
Iṣe Hecin reagent lodi si Ọna Comparator lori gbogbo awọn apẹẹrẹ ẹjẹ.
Ilana Igbeyewo
Iwe-ẹri Iforukọsilẹ
JT08-1T
JT08-5T
JT08-50T
